Peptide mapping is an important technique for the comprehensive characterization of biopharmaceutical products. In a peptide map, each peptide fragment must be resolved into a single peak, which is a significant chromatographic challenge due to the inherent complexity of protein digests.
In addition to the large number of peptide fragments that are generated from the enzymatic digestion of a protein, the number of alternative peptide structures (post-translational modifications, oxidations, etc.) can be equally difficult. Achieving adequate resolution in peptide maps is a tedious process. Good methods often have long run times and may not be as rugged as desired. These factors have all been addressed with the UPLC® Peptide Analysis Solution.
With this specialized system configuration, you benefit from:
- Enhanced resolution, reproducibility, and sensitivity
- Qualitative and quantitative analysis of variant peptides with chromatographic resolution and undistorted peak shape at extreme molar ratios
- Wide dynamic range ensuring that peptides at extreme molar ratios are detected even at the lowest limits of quantitation
- Flexibility in peptide mapping, essential for characterizing proteins
- Choices for method development with columns that are quality control tested and certified using a peptide digest to ensure reproducibility
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