HILIC Column Technology and Its Applications for LC Analysis

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Date(s) - August 16, 2022
8:30 am - 12:00 pm


Among the many stationary phase chemistries available for hydrophilic interaction chromatography (HILIC), zwitterionic materials employing sulfobetaine groups have proven to have the broadest utility. Incorporating negatively and positively charged moieties in a one-to-one molar ratio, zwitterionic stationary phases exhibit strong retention and a net zero surface charge.


Here we describe a new zwitterionic sulfobetaine stationary phase based on ethylene-bridged hybrid (BEH) organic/inorganic particles, offering a wider pH range (2–10) than silica-based zwitterionic materials and higher efficiencies compared to organic polymer-based zwitterionic columns. We demonstrate the efficiency, retention, selectivity, batch-to-batch reproducibility, and pH stability of this material. We also show the excellent performance of columns packed with this material for metal-sensitive analytes, which is enabled by the MaxPeak High Performance Surfaces column hardware.



■ High column efficiencies

■ Strong retention for neutral polar analytes

■ Complementary selectivity to BEH Amide and BEH HILIC columns

■ Excellent stationary phase batch-to-batch reproducibility

■ Stable over a wide pH range (2–10) for method flexibility

■ Excellent recoveries and peak shapes for metal-sensitive analytes



Speakers and Topic :

 Shalene Goh

Chemistry Business Development Specialist Waters Corporation.

Premier HILIC Solution to Solve Challenges of the Separation of Polar Compounds.


Satya Ibnu Purnamahadi

Product Development Manager Kromtekindo Utama

Adressing Small Molecule Challenges with the ACQUITY PREMIER Solution


Fasilitas :

  • Soft File Materi


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☎️ Muhammad Galih Ekaputra : 08568435525

☎️ Tiara Avinti Andani : 087777738300

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